raw western blots and imaging data v1  (Mendeley Ltd)

Journal: iScience

Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

doi: 10.1016/j.isci.2025.112458

Figure Lengend Snippet: Akk improves hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A–C) Akk significantly affected mouse liver protein network interaction pathways, significantly regulated pathways and differential proteins by proteomic analysis. (D–J) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (K) mRNA levels of genes related to lipogenesis (ACC1, FASN, SCD1, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.

Article Snippet: Raw data of Western Blot Data , This paper , Mendeley Data, https://doi.org/10.17632/3fzfd2npw8.1.

Techniques: Western Blot

Journal: iScience

Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

doi: 10.1016/j.isci.2025.112458

Figure Lengend Snippet: α-ketoisovaleric acid is the key metabolite in improving hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A) Flowchart of α-ketoisovaleric acid compensation experiment. All mice, except for the Con group, were fed an HFHCD from week 1 to week 8. α-ketoisovaleric acid group was orally administered α-ketoisovaleric acid every day, while the Con and Mod groups were given saline. (B) Representative photographs of mouse livers from each group. (C–H) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (I) mRNA levels of genes related to lipogenesis (ACC1, FASN, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. (J) Akk improves hepatic lipid metabolism via PI3K/Akt pathway through α-ketoisovaleric acid. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA. Scale bars: 1 cm.

Article Snippet: Raw data of Western Blot Data , This paper , Mendeley Data, https://doi.org/10.17632/3fzfd2npw8.1.

Techniques: Saline, Western Blot

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Mof and H4K16ac expressions were down-regulated in DIO livers (A–C) The livers of WT and DIO mice were subjected to Oil-O, PAS, and HE staining. Scale bar: 100 μm. See also Figure S4 A. (D and E) Impaired glucose and insulin tolerance in DIO mice were demonstrated through IPGTT and IPITT (n = 10). (F–H) Disturbance in lipid metabolism was indicated by measurements of serum TG, cholesterol, and intra-hepatic TG (n = 5). (I and J) Hepatic damage was reflected by levels of ALT and AST (n = 6). (K–M) Decreased Mof and H4K16ac levels, as well as impaired amino acid, glucose, lipid metabolism, and autophagy, were observed through western blotting (n = 3). GAPDH was the loading control, with quantification in the right panel. (N) Decreased Mof expression in DIO mice were demonstrated through IHC staining. Scale bar: 20 μm. (O) Reduced H4K16ac level in DIO mice were demonstrated through IF staining. Scale bar: 20 μm. Graph data were presented as mean ± SEM. In D–J, n represents the number of mouse. In K–M, n represents the number of western blotting data. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Staining, Western Blot, Control, Expressing, Immunohistochemistry

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Mof knockdown contributed to hepatic metabolic alterations (A) The flow-chart illustrating the deficiency of Mof induced by 4-OHT. (B) The results obtained from western blotting demonstrated the knockdown of Mof and decreased levels of PPARɑ and H4K16ac (n = 3). (C and D) The levels of serum and intrahepatic TG were measured (n = 3). (E and F) The levels of cholesterol were measured (n = 3). (G and H) The levels of alanine were measured (n = 3). (I) The level of ALT was measured (n = 3). (J and K) PAS staining and Oil-O staining revealed increased lipid accumulation and decreased glycogen storage in the livers deficient in Mof. Scale bar: 50 μm. See also Figure S4 B. Graph data were presented as mean ± SEM. n represents the number of mouse. ∗p < 0.05, ∗∗p < 0.01 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Knockdown, Western Blot, Staining

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Mof played distinct roles in lipid metabolism via PPARα or mTOR signaling pathway under different circumstances (A) AML 12 cells were subjected to Oil-O staining following treatment with either DMSO or mg149. Scale bar: 50 μm. See also Figure S4 C. (B) Oil-O staining was performed on AML 12 cells treated with PA + DMSO or PA + mg149. Scale bar: 50 μm. See also Figure S4 D. (C) The effect of mg149 on H4K16ac, PPARɑ, LC3, and mTOR was assessed using western blotting (n = 3). GAPDH was the loading control, with quantification in the right panel. (D) Western blotting analysis revealed the influence of PA on Mof, H4K16ac, p -mTOR, mTOR, and PPARɑ (n = 3). GAPDH was the loading control, with quantification in the right panel. (E) Western blotting demonstrated the impact of mg149 on mTOR in AML 12 cells treated with PA (n = 3). GAPDH was the loading control, with quantification in the right panel. Graph data were presented as mean ± SEM. n represents the number of western blotting data. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Staining, Western Blot, Control

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on wild-type hepatic metabolism (A and B) IPGTT and IPITT of untreated WT mice (n = 7). (C and D) IPGTT and IPITT of DMSO or mg149 administrated WT mice (n = 7). (E) Western blotting showed that mg149 treatment led to decreased hepatic PPARα and H4K16ac expressions (n = 4). (F) Measurements of serum TG levels of WT + DMSO and WT + mg149 groups (n = 7). (G) Measurements of intra-hepatic TG levels of WT + DMSO and WT + mg149 groups (n = 6). (H) Measurements of serum cholesterol levels of WT + DMSO and WT + mg149 groups (n = 7). (I) Measurements of intra-hepatic cholesterol levels of WT + DMSO and WT + mg149 groups (n = 7). (J) Measurements of serum alanine levels of WT + DMSO and WT + mg149 groups (n = 7). (K) Measurements of intra-hepatic alanine levels of WT + DMSO and WT + mg149 groups (n = 6–7). (L–M) ALT and AST between WT + DMSO and WT + mg149 groups showed no difference (n = 6–7). (N) Biochemical analysis showed that in mg149-treated WT mice, liver glycogen level was decreased (n = 7). (O) Oil-O staining indicated more lipid deposits in mg149-administrated WT mice. Scale bar: 50 μm. See also Figure S4 F. (P) PAS staining indicated less glycogen in mg149-administrated WT mice. Graph data were presented as mean ± SEM. n represents the number of mouse. ∗p < 0.05, ∗∗p < 0.01 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Inhibition, Western Blot, Staining

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on DIO hepatic biochemical analysis (A and B) IPGTT and IPITT of untreated DIO mice (n = 6 in DIO+DMSO group and n = 10 in DIO+mg149 group). (C and D) IPGTT and IPITT of DMSO or mg149 administrated DIO mice (n = 6 in DIO+DMSO group and n = 10 in DIO+mg149 group). (E) Western blotting showed that mg149 treatment led to decreased hepatic LC3 and H4K16ac expressions (n = 4). (F)Measurements of serum TG levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (G) Measurements of intrahepatic TG levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (H) Measurements of serum cholesterol levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (I) Measurements of intra-hepatic cholesterol levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (J) Measurements of serum alanine levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 8 in DIO+mg149 group). (K) Measurements of intra-hepatic alanine levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (L and M) ALT (p = 0.0769) and AST (p = 0.7156) between DIO+DMSO and DIO+mg149 groups showed no difference (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (N) Glycogen measurement indicated no difference in glycogen storage (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). Graph data were presented as mean ± SEM. n represents the number of mouse. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Inhibition, Western Blot

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on DIO hepatic morphological analysis (A) HE staining showed smaller lipid droplets in mg149 administrated DIO mice. Scale bar: 50 μm. (B) Oil-O staining showed less lipid deposit mg149 administrated DIO mice. Scale bar: 50 μm. See also Figure S4 G. (C) PAS staining indicated no difference in glycogen storage. Scale bar: 50 μm. (D) Western blotting showed decreased p -AKT and p -mTOR levels (n = 4 in DIO+DMSO group and n = 7 in DIO+mg149 group). (E) IHC staining showed decreased p -AKT levels. Scale bar: 50 μm. (F) IHC staining showed decreased p -mTOR levels. Scale bar: 20 μm. Graph data were presented as mean ± SEM. n represents the number of mouse. ∗∗p < 0.01 (Student’s t test).

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Inhibition, Staining, Western Blot, Immunohistochemistry

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet:

Article Snippet: Raw data of some western blotting , This paper , Mendeley Data: https://doi.org/10.17632/d6rztmt6gd.1.

Techniques: Labeling, Affinity Purification, Recombinant, Injection, Lysis, Staining, Transfection, Cell Culture, Modification, Cholesterol Assay, Polymer, Enzyme-linked Immunosorbent Assay, Western Blot, SYBR Green Assay, Knockdown, Mass Spectrometry, Sequencing, Software